Figure 3 | Scientific Reports

Figure 3

From: 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination

Figure 3

Immunohistochemical staining of SHH medulloblastoma 3D spheroids for markers of proliferation and hypoxia. DAOY and ONS76 3D spheroids were harvested on days 7, 14, and 21, sectioned, and stained with markers of (a) proliferation (Ki67) and (b) hypoxia (CA9) (scale bar 100 µm). For both cell lines, Ki67 expression was visible throughout the 3D spheroid on days 7 and 14, but expression was restricted to the periphery by day 21. CA9 core expression was present by day 14 and became more extensive by day 21.

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