Figure 4
From: Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry
Flow cytometric studies of HT29 cell suspensions with and without 100 µg/g doxorubicin exposure and/or 30 min of 47 °C hyperthermia. Viability analysis performed using L/D stain, assayed at 2, 6, 48 and 72-h (Columns 1–4). Rows 1–4: Side Scatter (SSC) vs. Forward scatter (FSC) with population of likely viable cancer cells selected by a circle, expressed percentage of all events centrally within the circle. No events discarded. Rows 5–8: FSC vs. far-red FL-4 channel (633/660 ± 16 nm) (see methods). 10,000 events recorded and events with FSC < 100 AU discarded, presumed to represent cell debris and/or impurities. Percentage of cell viability (top left) vs. death (top right) are demonstrated within the larger (outer) gated areas. The events within the viable cell gate with FSC between 100 and 200 AU are presumed to represent apoptotic bodies, and these have been excluded using a smaller sub-gate, with percentage of all events within that gate just above the FSC = 200 AU line. Plots generated using FlowJo Data Analysis Software.
