Figure 5
From: Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry
Summary plot of cell viability analysis for HT29 cells exposed to insults of either high dose doxorubicin (100 µg/mg) and/or 47 °C hyperthermia with controls, using L/D viability stain. Cells assayed at 2, 6, 24 48 and 72-h. There is more rapid and near-complete cell death in the group receiving both insults compared with the groups receiving a single insult, and minimal cell death is demonstrated in the control. Of note, if debris particles considered to be ‘apoptotic bodies’ are excluded, the results indicate near-total cell kill in the group receiving both insults at 72-h (blue dotted line).
