Figure 6
From: Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry
Cell viability flow cytometric studies of cells recovered from control liver and liver exposed to varying durations of hyperthermia and powers of HIFU. Each condition was performed in triplicate, i.e. three bags with liver sample exposed to hyperthermia and three liver lesions were performed and sampled individually at each HIFU power (N = 3, Columns). Representative plots for 0, 60, 120 and 240 min of hyperthermia (rows 1–4) and medium (7.27 MPa) and high (8.19 MPa) HIFU powers (rows 5–6) shown using FSC vs. far-red FL-4 channel (633/660 ± 16 nm). Events with FSC < 70 and/or FL-4 < 300 discarded, presumed to represent cell debris and/or impurities. Viability assessed using L/D stain. Trend for increased L/D staining intensity with samples receiving increasing insults, most markedly in the high power HIFU group, compared to the control group. Plots generated using FlowJo Data Analysis Software.
