Figure 7
From: Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry
Cell viability analysis for ex vivo bovine liver experiments. (a) Mean cell viability plot of cells recovered from control liver and liver exposed to varying durations of hyperthermia (45 °C) assayed at 16 h (N = 3). One-way ANOVA with Fisher’s LSD revealed significant increase in cell death (p < 0.01) for the groups treated with 60, 120 and 240 -min of hyperthermia when compared to the control, 15- and 30-min groups in any combination for both PI and L/D stains (**). (b) Cell granularity analysis for the same (hyperthermia) group (N = 3). One-way ANOVA with Fishers LSD revealed a significant increase (p < 0.01) in granularity (SSC) for the 60-to-240-min hyperthermia exposure groups when compared to the control. (c) Mean cell viability plot of cells recovered from control liver and HIFU-ablated lesions fixed at 4 h and assayed the following day (N = 3). One-way ANOVA with Fisher’s LSD revealed significant increase in cell death (p < 0.01) in both the medium and high-power groups compared to both the control group in any combination for the L/D stain (*). (d) Cell granularity analysis for the same (HIFU) group (N = 3). One-way ANOVA with Fishers LSD revealed a significant increase (p < 0.01) in granularity (SSC) for the medium and high HIFU exposure groups when compared to the control.
