Figure 8
From: Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry
SSC and FSC flow cytometric studies of cells recovered from control liver and liver exposed to varying durations of hyperthermia and powers of HIFU (N = 3, Columns). Representative plots for 0, 60, 120 and 240-min of hyperthermia (rows 1–4) and medium (7.27 MPa) and high (8.19 MPa) HIFU powers (rows 5–6) shown. Sub-populations have been gated, with gates labelled in top-left plot only. Plots demonstrate a trend for at first increased FSC (moving from R1 to R2), followed by dramatically increased SSC and FSC (cells moving from R1/R2 populations to R4) with increasing severity of hyperthermic or ablative insults, consequent with larger cell size and granularity. Events with FSC < 70 discarded. Plots generated using FlowJo Data Analysis Software.
