Figure 3
From: Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
(A) Schematic diagram showing the flow from on-site cell metabolomics conducted in the rice endosperm cells growing under controlled environments. After cell sap collection using a cell pressure probe (Step 1), the tip was immediately rotated (Step 2) and then picoPPESI-MS was performed under the same environements (Step 3) (also see “Methods” section). Scale bar in the transverse section embedded in A is 200 μm. (B–E) Negative ion picoPPESI mass spectra obtained from the cells in control and HNT treatment at 9 DAH daytime and nighttime. Inset figure in B indicates magnified range of m/z 200–350 to show peaks of [Hex + Cl]−, [d-Glucuronic acid + Cl]−, [HexP-H]−, [Glutathione-H]−, and [Hex2-H]−. The data are representative of repeated experiments with 9–14 kernels in each treatment.
