Figure 2 | Scientific Reports

Figure 2

From: Safety and efficacy of combination of suberoylamilide hydroxyamic acid and mitomycin C in reducing pro-fibrotic changes in human corneal epithelial cells

Figure 2

Effect of MMC and SAHA on cornea. Donor corneas were incubated for 1 month post surgery with MMC and SAHA. Representative immunofluorescence images of αSMA (red) and DAPI (blue) staining after MMC (A) and SAHA (B) treatment. Negative control for immunofluorescence images are corneas treated with MMC (C) and SAHA (D) and stained with secondary antibody only. Representative image of cornea cultured for 1 month without SAHA or MMC treatment and stained for αSMA (red) and DAPI (blue) (E). The white arrow heads show the αSMA positive staining, the yellow arrow head shows the stromal region and the green arrow depicts the endothelial layer of the cornea. mRNA expression of corneas (n = 4) treated with MMC (0.005%) and SAHA (5 µM) for 1 week and 1 month for αSMA levels (G). The mRNA levels of the gene of interest were normalized with the expression levels of β-actin gene. Mann- Whitney test for independent samples were run to get statistical significance. Images were quantified using Image J 1.48 version software (http://imagej.nih.gov/ij/) and statistical analysis performed using statistical software GraphPad PRISM Ver 6.01.

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