Table 1 Primers used in RT-qPCR.
Gene primer & Accession no | Forward sequence | Reverse sequence |
|---|---|---|
gapdh NM_001289746.1 | ACCCACTCCTCCACCTTTGAC | TGTTGCTGTAGCCAAATTCGTT |
bax NM_138761 | TTGCTTCAGGGTTTCATCCA | AGACACTCGCTCAGCTTCTTG |
bcl2 NM_ 000633 | TGGCCAGGGTCAGAGTTAAA | TGGCCTCTCTTGCGGAGTA |
αSMA NM_001141945.1 | GCTGGCATCCATGAAACCAC | TACATAGTGGTGCCCCCTGA |
TGFβ NM_000660.5 | CAGCAACAATTCCTGGCGATACCTC | CAACCACTGCCGCACAACTC |
Lox NM_002317.5 | ACATTCGCTACACAGGACATC | TTCCCACTTCAGAACACCAG |
Col4A1 NM_001845.5 | GCAAACGCTTACAGCTTTTGG | GGACGGCGTAGGCTTCTTG |
Il6 NM_000600.3 | GATGAGTACAAAAGTCCTGATCCA | CTGCAGCCACTGGTTCTGT |
Decorin NM_ 001920.3 | GCTTCTTATTCGGGTGTGAGT | TTCCGAGTTGAATGGCAGAG |
Fibronectin NM_ 212482.1 | TGGCCAGTCCTACAACCAGTA | |
Collagen 3A1 NM_ 000090.3 | ||
abcg2 NM_004827 | GAGCCTACAACTGGCTTAGACTCA | TGATTGTTCGTCCCTGCTTAGAC |
p63α NM_001114980 | AGCCAGAAGAAAGGACAGCA | CAGGTTCGTGTACTGTGGCT |
Ck3 NM_057088 | AGTTTGCCTCCTTCATTGACA | TGCCTGAGATGGAACTTGTG |
Ck12 NM_000223 | ACGAGCTGACCCTGACCA | CGGAAGCTTTGGAGCTCAT |
Cyclin D1 NM_002592 | AGCTCCTGTGCTGCGAAGTGGAAAC | AGTGTTCAATGAAATCGTGCGGGGT |
Ki67 NM_002417 | CTTTGGGTGCGACTTGACG | GTCGACCCCGCTCCTTTT |
