Table 2 Details of antibodies used in immunofluorescence.
Primary antibodies | Dilution | Vender |
|---|---|---|
αSMA, mouse | 1:50 (IF); 1:1000 (WB) | Abcam, USA |
TGFβ, rabbit | 1:50 (IF) | Santacruz Biotechnology, USA |
Collagen 4, rabbit | 1:50 (IF): 1:1000 (WB) | Abcam, USA |
BCL2, rabbit | 1:250 (IF); 1:1000 (WB) | Abgenex, India |
CK3/12, rabbit | 1:1000 (WB) | Abcam, USA |
ABCG2-APC, mouse | 1:20 (FACS) | Biolegend, USA |
APC-IgG2b, κ isotype, mouse | 1:20 (FACS) | Biolegend, USA |
P63α, rabbit | 1:1000 (WB) | Cell Signaling, USA |
GAPDH, mouse | 1:1000 (WB) | Abgenex, India |
Secondary antibodies | ||
Donkey anti-rabbit IgG, Cy3 | 1:500 | Jackson Immunoresearch, USA |
Donkey anti-rabbit IgG, Alexa flour 488 | 1:500 | Abgenex, India |
Donkey anti-mouse IgG, Cy3 | 1:500 | Jackson Immunoresearch, USA |
Donkey anti-rabbit IgG, HRP | 1:4000 | Biolegend, USA |
Goat anti-mouse IgG, HRP | 1:4000 | Biolegend, USA |
