Table 1 MicroRNA in situ hybridization, ISH, result table.

From: A novel approach for microRNA in situ hybridization using locked nucleic acid probes

Fixation time (h)

Time (min)

Cervix

Prostate

Tonsil

Placenta

Statistics

5

30

100

24

72

144

6

48

120

6

36

140

Mean 1

SD 1

ICC 1

p value 1

Global

Proteinase K

2.5

0.6

1.4

1.9

1.4

1.7

2.5

0.7

1.7

2.2

0.3

0.7

1.4

1.4

0.9

0.9

8.0e−03

0.9

0.9

2.9e−23

Proteinase K

5

0.3

0.9

1.7

1.1

1.5

2.2

0.4

1.3

2.3

0.0

0.4

1.7

1.1

0.8

0.9

6.0e−05

Proteinase K

10

0.2

0.4

1.3

0.8

1.0

1.6

0.2

0.3

1.3

0.0

0.4

0.9

0.7

1.0

0.9

1.0e−10

Proteinase K

20

0.1

0.0

0.3

0.5

0.5

1.2

0.0

0.1

0.3

0.0

0.0

0.0

0.2

0.3

0.9

1.1e−20

TRS pH 6.1

20

03

0.6

1.7

0.6

0.9

1.8

0.4

0.9

1.8

0.1

0.2

0.9

0.9

0.4

0.9

1.3e−08

1.1

0.8

1.1e−14

TRS pH 6.1 + pepsin

20 + 3

0.4

0.8

1.7

0.7

1.3

1.9

0.7

1.2

2.1

0.0

0.2

1.4

1.0

0.2

0.9

2.1e−06

TRS pH 6.1 + pepsin

20 + 5

0.8

0.8

2.3

1.0

1.4

2.3

0.6

1.1

2.1

0.2

0.2

1.6

1.2

0.3

0.8

5.0e−04

TRS pH 9

20

1.2

1.4

2.8

1.5

2.0

2.8

1.3

1.8

2.7

0.3

0.5

2.0

1.7

0.3

0.9

5.6e−01

1.8

0.9

–

TRS pH 9 + pepsin

20 + 3

1.4

1.3

2.8

1.4

1.8

2.6

1.3

1.8

2.6

0.3

0.7

2.3

1.7

0.3

0.9

4.2e−01

TRS pH 9 + pepsin

20 + 5

1.4

1.6

2.9

1.4

1.9

2.6

1.3

2.2

2.9

0.3

0.8

2.5

1.8

0.1

0.8

–

Mean 2

0.7

0.9

2.0

1.0

1.4

2.2

0.7

1.2

2.0

0.1

0.4

1.5

 

SD 2

0.6

0.7

0.9

0.7

0.8

0.9

0.5

0.8

0.9

0.2

0.5

0.8

 

ICC 2

0.9

0.8

0.7

0.9

0.8

0.8

0.9

0.9

0.7

0.9

0.7

0.2

 

p value 2

7.9e−14

6.1 e−14

2.5 e−02

4.7 e−14

1.0 e−13

–

1.5 e−14

2.1 e−10

2.4 e−01

7.4 e−17

2.2 e−16

9.7 e−11

 
  1. The means of all three ISH experiments with the miR-205-5p, miR-145-5p and miR-126-3p probes, due to the given parameters, are presented within the largest square (main table). The upper horizontal bar shows the included tissue, and the numbers indicates the period of formalin fixation in hours. The first column from the left indicates pretreatment for permeabilization: proteinase K or Heat-induced microRNA Retrieval (target retrieval solution (TRS) pH 6.1 or TRS pH 9) with (w/) or without (w/o) pepsin addition. The second column shows exposure time for the given treatment. The additional number after the (+) presents enzymatic treatment w/ pepsin in minutes (min). The five columns to the right and four rows below the main table display statistics for the wide and longitudinal data: each test according to the ten pretreatments, regardless of tissue type and fixation period or the twelfth tissue-fixation categories. Mean 1 and 2 presents the mean across data points for the given pretreatment or tissue-fixation category SD 1 and 2, standard deviation. ICC 1 and 2, Intraclass correlation coefficients. p value 1 and 2, pairwise Wilcoxon signed-rank test relative to the best rated, assigned as (−). Global, the statistics are gathered into three categories: (1) Proteolysis; proteinase K (2.5–20 min), (2) TRS pH 6.1 (w/ and w/o pepsin) and (3) TRS pH 9 (w/ and w/o pepsin). Thus, the first number is the mean. The second is standard deviations. The third is p value of pairwise Wilcoxon signed-rank test relative to the best rated category, assigned as (−).
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