Table 2 Overall result table.

Fixation	Category	Mean	p values	Short	Moderate
5–24 h	Short	0.64	Moderate	4.3e−16	–
30–76 h	Moderate	0.99	Moderate	4.3e−16	–
100–144 h	Long	1.91	Long	3.5e−47	1.6e−44

Permabilization	Category	Mean	p values	Proteolysis	TRS pH 6.1
Proteolysis	Proteinase K	0.9	TRS pH 6.1	2.9e−03	–
TRS pH 6.1	w/w/o pepsin	1.1	TRS pH 6.1	2.9e−03	–
TRS pH 9	w/w/o pepsin	1.8	TRS pH 9	2.9e−23	1.1e−14

The first column to the left presents the fixation time given in hour intervals and permeabilization categories: Proteolysis, Target Retrieval Solution (TRS) pH 6.1 and TRS pH 9. Second column, category of hour interval: short (5–24 h), moderate (30–76 h), long (100–144 h) and category of permeabilization: proteinase K or with (w/) or without (w/o) pepsin. Third column, Mean: the mean of the scores given in each category (Category). The fourth to the sixth column: a pairwise comparison of all categories using a Wilcoxon rank sum test. The fourth column and upper bar of column six and five displays the category to be compared (short, moderate or long). The numbers in column five and six display the p value of the given comparison of the categories. The p values were adjusted using the Benjamini and Hochberg procedure.

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