Figure 5 | Scientific Reports

Figure 5

From: Pathologically phosphorylated tau at S396/404 (PHF-1) is accumulated inside of hippocampal synaptic mitochondria of aged Wild-type mice

Figure 5

Tau PHF-1 accumulates inside hippocampal synaptic mitochondria at an advanced age. (a) Immuno-gold for PHF-1 tau in the hippocampal CA1 region of 3 and 18 month-old mice. Representative images of immuno-gold electron microscopy (IEM) (43,000x) showing (i) the amount of PHF-1 positive signal within an area of the grid of 7 mm2; (ii) amount of PHF-1 in the mitochondria, (iii) the number of PHF-1 tau in pre and (iv) post-synaptic mitochondria. (b) Quantitative analysis of IEM. (c). Western Blot of the proteinase K protection assay in a mitochondrial fraction of 18 mo mice. The mitochondrial fraction was treated with Proteinase K with or without 0.1% Digitonin or 1% Triton X-100. The mitochondrial fraction without Proteinase K was used as a control. (left, Assay 1) Western Blot of the total volume of mitochondrial samples. (Right, Assay 2) Western Blot of 1/3 of the volume of samples 1 and 2. n = 4 different animals. Graph bars represent means ± SEM. *p < 0.05, **p < 0.01.

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