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Figure 1

From: Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

Figure 1

Relative expression of target genes determined by REST 2009 (n = 3). The error bars represent standard error. a.u: arbitrary units. P < 0.001 (***), P < 0.01(**), and P < 0.05(*). Abbreviations: ML7th Mature leaf 7th time point, ML13th Mature leaf 13th time point, SL7th Spindle Leaf 7th time point and SL13th Spindle Leaf 13th time point, SD short day. (A) PIL5 normalized with most stable reference genes (UBQ1/TUB), (B) PIL5 normalized with least stable reference genes (GAPDH/25SrRNA1), (C) LHP1 normalized with most stable reference genes and (D) LHP1 normalized with least stable reference genes.

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