Table 2 Coefficient of variation (CV) according to BestKeeper algorithm, for tissue type, developmental stage (7th and 13th time points) and photoperiod treatment.

From: Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

Rank

Tissue type

Developmental stage

Photoperiod treatment

Mature Leaf

Spindle Leaf

7th time point

13th time point

SD

LD

Gene

CV

Gene

CV

Gene

CV

Gene

CV

Gene

CV

Gene

CV

1

RPL

3.49

UBQ2

6.18

EF1

3.00

25SrRNA1

1.48

UBQ2

1.56

UBQ1

5.18

2

UBQ2

4.70

UBQ1

8.14

TIPS-41

3.40

UBQ1

1.78

RPL

3.50

RPL

6.44

3

TUB

5.41

RPL

8.32

RPL

3.64

EF1

4.52

TIPS-41

7.30

UBQ2

7.60

4

TIPS-41

6.20

EF1

9.72

UBQ2

5.22

UBQ2

4.76

TUB

7.52

TIPS-41

8.41

5

UBQ1

6.37

TIPS-41

9.91

UBQ1

5.23

TIPS-41

5.95

UBQ1

9.34

EF1

10.62

6

GAPDH

11.34

GAPDH

13.27

25SrRNA1

5.71

TUB

7.19

GAPDH

10.05

TUB

13.55

7

EF1

11.76

TUB

13.59

TUB

10.12

RPL

7.54

EF1

11.17

GAPDH

14.80

8

25SrRNA1

22.60

25SrRNA1

24.07

GAPDH

13.64

GAPDH

8.44

25SrRNA1

25.55

25SrRNA1

21.13

  1. SD short day; LD long day.