Table 3 Summarized data of the reference genes highlighted by the NormFinder and BestKeeper algorithms in different experimental samples.

From: Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

Algorithms

BestKeeper

NormFinder

Experimental samples

Gene symbol

Standard deviation

Coeff. of corr.[r]

P value

Gene symbol

Stability

Mature leaf

Most stable gene

RPL

0.94

0.88

0.004

TUB

0.02

Least stable gene

GAPDH

2.91

0.18

0.669

25SrRNA1

0.18

Best pair

N.A

N.A

N.A

N.A

RPL/EF1

0.02

Spindle leaf 

      

Most stable gene

UBQ2

1.53

0.92

0.001

TIPS-41

0.04

Least stable gene

RPL

2.25

0.82

0.013

25SrRNA1

0.15

Best pair

N.A

N.A

N.A

N.A

UBQ1/GAPDH

0.01

7th time point

      

Most stable gene

RPL

0.96

0.99

0.001

RPL

0.01

Least stable gene

UBQ2

1.27

0.34

0.418

GAPDH

0.09

Best pair

N.A

N.A

N.A

N.A

UBQ1/TUB

0.02

13th time point

      

Most stable gene

EF1

1.59

0.91

0.002

UBQ2

0.02

Least stable gene

UBQ1

0.65

 − 0.76

0.03

25SrRNA1

0.06

Best pair

N.A

N.A

N.A

N.A

RPL/EF1

0.02

SD

      

Most stable gene

RPL

0.91

0.90

0.002

UBQ1

0.03

Least stable gene

GAPDH

2.48

0.35

0.393

25SrRNA1

0.20

Best pair

N.A

N.A

N.A

N.A

RPL/EF1

0.03

 LD 

      

Most stable gene

UBQ2

1.97

0.89

0.003

TIPS-41

0.05

Least stable gene

25SrRNA1

4.25

0.98

0.001

25SrRNA1

0.12

Best pair

N.A

N.A

N.A

N.A

UBQ1/TUB

0.02

  1. N.A Not applied to this algorithm. Complete data is available in Supplementary Table S2 online. Coeff.of corr. [r] stands for Pearson correlation between Bestkeeper index and candidate reference genes.
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