Figure 3 | Scientific Reports

Figure 3

From: Fast visual exploration of mass spectrometry images with interactive dynamic spectral similarity pseudocoloring

Figure 3

Result of the matrix signal subtraction step: Three average mass spectra normalized to [0,1] of the human PXE skin data set (\(I^\text {t}_\text {s}\)) measured with DHB matrix and MALDI-ToF are shown in the center column. The topmost mass spectrum shows the average spectrum before matrix subtraction. The middle MS is the approximate average spectrum of the matrix pixels manually selected in the matrix subtraction tool of ProViM. The main matrix mass range is highlighted with a blue box. Three representative mass channels are marked with arrows and their corresponding single mass channel images with matrix specific distributions are shown in the left column. The lowest MS shows the overall average spectrum after matrix subtraction with ProViM. The lipid region is highlighted with a green box and three representative mass channels are marked with arrows. The corresponding mass channel images with tissue-specific distributions are shown in the right column. ProViM matrix subtraction substantially enhances the mass signals of the lipid region of interest.

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