Figure 7

P110 reverses propofol-induced SO production and mitochondrial fragmentation. Representative cropped fluorescent images showing neurons from each condition which are co-stained with MitoTracker Green FM for mitochondria and the SO indicator MitoSOX Red (A). Quantification of predominant mitochondrial morphology in each treatment (B). For the ease of comparison, the control and anesthetic alone data are recapitulated here. P110 alone did not affect mitochondrial morphology whereas P110 pre-treatment fully rescued against propofol and ketamine-induced fragmentation. Quantification of relative SO production. For the ease of comparison, the control and anesthetic alone data are recapitulated here (C). P110 alone did not affect SO production, however, P110 pre-treatment completely protected against propofol-induced SO production but not ketamine-induced SO production. Quantification of relative SO based on MitoSOX Red signal which colocalized to MitoTracker Green FM (D). For the ease of comparison, the control and anesthetic alone data are recapitulated here. P110 reversed propofol-induced elevation in mitochondrial SO production but not ketamine-induced changes. Two-Way ANOVA. * p < 0.05, ** p < 0.01, **** p < 0.0001 as determined by pairwise comparison of post-hoc tests. Bars indicate ± SD across at least biological replicates. Each data-point indicates the either the percentage of cells that exhibited a specific morphology per dish, or the mean SO signal recorded per dish. Scale bars indicate 10 μm.