Figure 1
From: Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope
(A) Dot blot of heat-treated sEGFR detected by antibodies 528 and cetuximab. Each blotting image was a consecutive membrane strip without cropping. (B,C) Competitive Biacore assay between 528 and EGF (B) and between 528 and cetuximab (C) for binding sEGFR. sEGFR (100Â nM) was loaded onto the antibody-immobilized sensor chips with or without its competitor.
