Figure 4
From: Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma
MALDI-TOF mass spectra of rMT2-CDDP complex and purified rMT2 standard. Upper insert shows CLUSTAL multiple sequence alignment of rabbit rMT2 and hMT3. Amino acid from the sequences with asterisk (*) indicate 100% of similarity between rMT2 and hMT3. (A) MALDI-TOF spectra of rMT2 and (B) complex between rMT2 and CDDP after 24 h incubation in PBS (pH 7.5). Each spectrum was averaged from 2000 subspectra. In each mass spectrum, the hypothetical models of hMT3 complexed with Zn and Pt are shown. hMT3 protein sequence (P25713) was used to build a homology model based on template structure of rat MT2 (PDB ID 4MT2) via SwissModel server. Assuming that superior electronegativity of Pt ions replaced Zn in a similar fashion to Cd ions, the 3D structures of hMT3 Pt/Zn complexes are shown here. (A.1) Zn7-MT3 and (B.1) Pt2Zn5-hMT3 and (B.2) Pt3Zn4-hMT3. hMT3 is shown in blue ribbon and molecular surface HETATOM colours. Zn and Pt ions are shown in orange and green, respectably. (C) Venn diagram showing exclusiveness of proteins identified in proteomic signatures of parental UKF-NB-4 and UKF-NB-4 cells with up-regulated hMT3. (D) Table showing the numbers of 1481common proteins and the distribution of their regulation (UKF-NB-4-MT3 vs. UKF-NB-4, up-regulation-fold ratio > 2.5; down-regulation-fold ratio < 0.5; 2.5 > no significant differences > 0.5).
