Figure 2
From: Antisense oligonucleotide development for the selective modulation of CYP3A5 in renal disease
Cation-specific Restoration of CYP3A5*3/*3 mRNA in HEK293 cells, but not CYP3A5*1/*1 CV-1 cells. (a) Endpoint PCR monitoring of CYP3A5 mRNA expression in HEK293 cells exposed to: 1 (control), 2 (NaCl enriched), and 3 (LiCl enriched) media revealed cation-specific induction of alternative gene splicing. Several lanes were removed from this original gel image to aid in the molecular weight interpretation shown here, and this gap is denoted by the white dividing line. (b) SYBR Green qPCR Analysis of CYP3A5 mRNA from HEK293 (CYP3A5*3) and CV-1 (CYP3A5*1) cells incubated with media supplemented with increasing amounts of (4, 5, 7, and 14 mM) of KCl. Normal DMEM media contains 4 mM KCl.
