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Figure 1

From: Human BM-MSC secretome enhances human granulosa cell proliferation and steroidogenesis and restores ovarian function in primary ovarian insufficiency mouse model

Figure 1

Human mesenchymal stem cell conditioned media (hMSC CM) decreases cyclophosphamide-induced apoptosis of human granulosa cells (HGrC1). (a) Experimental design of the in vitro study. (b–e) Expression of apoptosis marker caspase 3 by immunoblot in cyclophosphamide-treated HGrC1 that were untreated (control, Group I) or treated with hMSC CM (Group II). Quantification of total caspase 3 (c), pro-caspase 3 (d), and cleaved caspase 3 (e) based on immunoblot protein band density. (f) Apoptosis marker gene expression in cyclophosphamide-treated control HGrC1 (Group I) and hMSC CM-treated HGrC1 (Group II). Two biological replicates per treatment group were analyzed in duplicate (*P < 0.05, **P < 0.01, N/S not significant).

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