Figure 2
From: Role of the V1G1 subunit of V-ATPase in breast cancer cell migration
V1G1 modulates invasive phenotype of breast cancer cells. V1G1-depleted MCF7 (V1G1i) (a) and HA-V1G1 overexpressing MDA-MB-231 (b) or Hs578T cells (c) were imaged during wound healing assay at initial time point (T0) and 8 h after the scratch. Cell migration was measured as the ratio between the closed area of the wound in control cells (HA or scr) and HA-V1G1 overexpressing or V1G1 silenced cells. Scale bar = 100 µm. (d) Gelatin zymography was performed using conditioned medium of MDA-MB-231 cells overexpressing HA-V1G1 and control. Representative results are shown. (e) Western blot analysis was performed using specific anti-MMP-2, anti-HA and anti-tubulin antibodies. Intensity of bands was measured using the software Image Lab. Data represent the mean ± s.e.m. of at least three experiments. Statistical analysis was performed using Student’s t-test with control cells (scr or HA) as referring sample set to 1. **p ≤ 0.01; ***p ≤ 0.001.
