Figure 4
From: Role of the V1G1 subunit of V-ATPase in breast cancer cell migration
V1G1 overexpression affects RAC1 signaling. (a) MDA-MB-231 cells overexpressing HA-V1G1 and control (HA) were lysed and subjected to western blot analysis using anti-HA, anti-RAC1, anti-cofilin and anti-tubulin antibody. Quantification of RAC1 and cofilin protein amount is shown. Data represent the mean ± s.e.m. of at least three experiments. **p ≤ 0.01; i***p ≤ 0.001. (b) HA-V1G1 overexpressing MDA-MB-231 cells were immunolabeled with anti-HA followed by Alexa488 conjugated secondary antibody to discriminate between cells overexpressing HA-V1G1 and control cells, anti-vinculin followed by Alexa568 conjugated secondary antibody while nuclei were stained with DAPI. Bars = 10 µm.
