Figure 5 | Scientific Reports

Figure 5

From: Cholesterol loading suppresses the atheroinflammatory gene polarization of human macrophages induced by colony stimulating factors

Figure 5

Effect of cholesterol loading on the gene expression of ABC transporters and related transcription factors in M-MØ and GM-MØ foam cells. M-MØ and GM-MØ were cholesterol-loaded for 24 h with 25 µg/mL of acetyl-LDL (ac-LDL), oxidized-LDL (ox-LDL), or native LDL. (A) Cholesterol was quantified by HPTLC, and expression of (B) ABCA1 and (C) ABCG1 was determined by quantitative RT-PCR. Data in panels (B) and (C) indicate mRNA expression levels relative to those in the non-loaded cells (Macrophages) (set at 1). Gene expression of (D) LXRA, (E) PPARA, and (F) PPARG was determined in acetyl-LDL generated foam cells of each subtype. Data in panels (D) to (F) indicate mRNA values expressed as arbitrary units. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA. Data are presented as means ± SEM of 4–9 individual blood donors and triplicate incubations for each loading condition and gene of interest.

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