Figure 1
From: Endothelin-1 mediated vasoconstriction leads to memory impairment and synaptic dysfunction
Decreased expression of CD31 after 3 days of bilateral ET-1 injection. (A) (i) Shows the site of injection on the atlas of Franklin and Paxinos (1997) of mouse brain depicting the location of cerebral ventricles where ET-1 was injected. (ii) The Nissil staining showing the needle track at the site of injection. (iii) The coronal sections of mouse brain show the dye spread from site injection. (B) The expression of CD31 was decreased after 3 days of ET-1 injection in CA1 region of hippocampus compared to vehicle control at ×10 magnification. The arrows show pyramidal layer in CA1 region of hippocampus and micro vessels. (C) The images are at 20x magnification of ET-1 injected and vehicle control used for quantitation (-ve control is used to indicate that there was no nonspecific staining). (D) The graph showing number and length of CD31 positive blood vessels at 3 days of ET-1 injection. Values are from three sections of hippocampus of control and ET-1 injected mice expressed as Mean ± SD of n = 3 animals with statistical significance.
