Figure 4
From: Endothelin-1 mediated vasoconstriction leads to memory impairment and synaptic dysfunction
Local activity-dependent protein translation at the synapse is diminished in ET-1 treated mice. (A) Electron micrograph of synaptoneurosomes indicating postsynaptic density and vesicles in presynaptic terminal. (B) Protein translation measured as S35-methionine incorporation in PNS fraction of hippocampus was unaffected in control and ET-1 treated mice. (C) Synaptoneurosomes prepared from hippocampus of control and ET-1 treated mice were subjected to protein translation after KCl stimulation was measured as S35-methionine incorporation. Stimulation of protein translation in synaptoneurosomes in the presence of KCl was abolished in ET-1 treated mice after 7 day of ET-1 treatment. Data are represented as Mean ± SD (n = 5) and values are significantly different from corresponding controls. (D) The graphical representation shows different players involved in Akt-mTOR pathway leading to protein translation.
