Figure 5
From: Endothelin-1 mediated vasoconstriction leads to memory impairment and synaptic dysfunction
Impairment in Akt1-mToR pathway phosphorylation in hippocampal lysate of bilateral ET-1 injected mice. (A) The levels phosphorylated forms of Akt1 Ser473 was decreased in ET-1 injected mice. However, there was no difference in total Akt1 form between ET-1 injected and vehicle control. (B) The graph represents the relative expression phospho Ser473 to total Akt1 and values are Mean ± SD, n = 8 animals. (E) Phosphorylation of Thr308, which are critical for kinase activity was decreased after 7 days of ET-1 treatment. (F) Shows the data of relative expression phospho Thr308 to total Akt1 and values are Mean ± SD, n = 8 animals. (I) Further phosphorylation of GSK was also low after 7 days of ET-1 treatment compared to total GSK. (J) Graph shows the values of Mean ± SD of n = 8 animals, values are significantly different from corresponding controls. (C) There was decrease in levels of phosphorylated mTOR2448 in ET-1 treated mice. (D) The graph represents relative expression phosphomTOR to total mTOR, values are Mean ± SD of n = 8 animals. (G) The downstream molecule of mTOR, pS6K at Thr389 was low in hippocampal lysate of bilateral ET-1 injected mice after 7 days of ET-1. (H) The data shows significant decrease between ET-1 treated and vehicle control. (K) The blot showed decreased expression of p4EBP at Thr46/47 after 7 days of ET-1 treatment. (L) The graph represents significant difference in relative expression of p4EBP to total 4EBP in ET-1 treated mice after 7 days.
