Figure 5
Characterization of phospholipid classes and fatty acid ratios of Huh-7 cells treated with fatty acids (PA or OA), SCD1 inhibitor or Tg, and of their released EVs. Panels A, C and E show data relative to EVs. Panels B, D and F show data relative to cells. (A, B) PC content (sum of all detected species) expressed as pmol/μg of proteins; ratio of PCs with acyl chains whose sum was < 36 carbons (short chain) to PCs acyl chains whose sum was > 38 carbons (long chains); ratio of saturated to unsaturated PC species. (C, D) PE content (sum of all detected species) expressed as pmol of lipid species/μg of proteins; ratio of PEs with acyl chains whose sum was < 36 carbons (short chain) to PEs with acyl chains whose sum was > 38 carbons (long chains); ratio of saturated to unsaturated PE species. (E, F) PS content (sum of all detected species) expressed as pmol of lipid species/μg of proteins; ratio of PSs with acyl chains whose sum was < 36 carbons (short chain) to PSs with acyl chains whose sum was > 38 carbons (long chains); ratio of saturated to unsaturated PS species. Mean values ± SD (n = 5, EVs; n = 3, cells) are shown (**p < 0.01, * p < 0.05, treated vs CTRL; ANOVA followed with a Dunnett’s multiple comparisons test).
