Figure 6
Effects of treatment with fatty acids (PA or OA), SCD1 inhibitor or Tg on the levels of SM and Cer in Huh-7 cells and their released EVs. Heatmap of clustering results showing the molecular species of SM and Cer significantly changed among all lipid species detected. Each rectangle represents a lipid colored by its normalized intensity scale from green (decreased level) to red (increased level). The Euclidean metric for distance measurement and the Ward.D algorithm for hierarchical clustering were used. Bar graphs show changes in the abundance of individual lipid species. Data are reported as mean ± SD (n = 3, cells; n = 5, EVs); p < 0.05 was considered statistically significant by one-way ANOVA and Tukey’s post-hoc analysis; columns with different letters are significantly different (i.e. a vs CTRL, b vs PA, c vs OA, d vs Tg, e vs SCD1i and π vs all).
