Table 2 Primary antibodies used in immunohistochemistry.

From: Epithelial to mesenchymal transition and microRNA expression are associated with spindle and apocrine cell morphology in triple-negative breast cancer

Biomarker

Type of primary antibody

Manufacturer

Concentration

ER

Mouse, monoclonal, 1D5

Dako; Agilent Technologies, Inc

1:20

PR

Mouse, monoclonal, PgR 636

Dako; Agilent Technologies, Inc

1:100

HER2

Rabbit, polyclonal, HercepTest™

Dako; Agilent Technologies, Inc

RTU

Vimentin

Mouse, monoclonal, V9

Dako; Agilent Technologies, Inc

1:10

E-cadherin

Rabbit, polyclonal,NCH-38

Dako; Agilent Technologies, Inc

1:50

GCDFP-15

Mouse, monoclonal, 23A3

Leica Biosystems

1:40

  1. RTU ready to use.
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