Figure 12
Cluster formation and biomarker expression of HepG2 embedded in shell compartment in monoculture (cell-free algMC core; I) and in co-culture with NIH 3T3 fibroblasts embedded in core compartment of different compositions (II-IV). (A) Confocal images of HepG2 stained for Albumin (purple), nuclei (blue) and cytoskeletons (green); scale bars 50 µm. (B) Confocal images of HepG2 stained for CK-19 (yellow), nuclei (blue) and cytoskeletons (red); scale bars 50 µm. (C) Confocal images of HepG2 stained for AAT (green), nuclei (blue) and cytoskeletons (red); scale bars 50 µm.
