Figure 3 | Scientific Reports

Figure 3

From: 3D bioprinting of hepatocytes: core–shell structured co-cultures with fibroblasts for enhanced functionality

Figure 3

Proliferation of HepG2 embedded in algMC with Matrigel – EdU proliferation assay. Cell nuclei were stained with DAPI (blue); representing original cells, while cells labelled with EdU are shown in green; representing newly formed DNA and therefore mitosis. Upper tile (20 × magnification) shows the distribution of newly formed cells allover a specific region of the scaffold. Lower tile (40 × magnification) shows distribution of cells within the formed clusters and their rate of proliferation; scale bars represent 100 µm.

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