Figure 3
From: Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
Evaluation of FBS substitution efficacy of SACCS. Cytotoxicity test of distilled water (DW), fetal bovine serum (FBS), 0.5× , 1× , 2 × , and 3 × spirulina animal cell culture solution (SACCS) on human lung cancer cell line H460 (A). Comparison of the cell morphology, metabolic proliferation activities, and cell viabilities of the cells in the control medium (FBS) and SACCS-substituted medium [1× SACCS (B, D) and 0.5× SACCS (C, E)]. Data are expressed as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the cells cultured in F10:S0. Bar = 100 μm. Figures were made with GraphPad Prism (ver. 8.1.1, San Diego, USA), https://www.graphpad.com/scientific-software/prism/.
