Figure 2
From: Removing auto-activators from yeast-two-hybrid assays by conditional negative selection
Quantification of auto activators in AD and BD and removal by 5-FOA treatment in liquid cultures. (A) Percentage of the auto-activators present in AD and BD libraries. The error bars indicate standard deviation. (B) Growth of the five AAs from the BD library on –L (left), –HULA, and –L + FOA (right). AA1-5 (rows) were spotted in three serial dilutions (1:1, 1:10, 1:100, columns) and grown for 3 days. (C) Growth of the five AAs from the BD library (red points) and three empty vector (EV, black points) controls in liquid –L (left), –LH, and –L + FOA (right).
