Figure 3 | Scientific Reports

Figure 3

From: Chromosomal translocation t(11;14) and p53 deletion induced by the CRISPR/Cas9 system in normal B cell-derived iPS cells

Figure 3Figure 3

Induction of differentiation of parent BiPSCs and their genome-edited BiPSCs into blood cells. (A) BiPSC13, (B) MIB2-6, (C) AX, (D) BG), (E) AZ, (F) BC, (G) AX with AID, and (H) G#28 with AID. (a) Flow cytometric analysis of the cell phenotype after differentiation of BiPSCs into HPCs. Two cell populations were observed (R1 and R2). (b) Representative morphology of formed colonies (× 50). (c) Wright staining of cytospins picked up from a colony (× 400). The bottom picture of (A) and arrows indicate erythrocytes. The bottom picture of (D) shows a mix of macrophages and erythrocytes. (I) Number of colonies formed. The types of colonies formed were assessed in triplicate.

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