Figure 1

SUN1 is associated with Golgi complex morphology. (a) HeLa cells were transfected with siSUN1 or siNC. After fixation, cells were stained with anti-GM130 mAb (green) and anti-SUN1 pAb (red). Note that, siSUN1 contains four siRNAs, siSUN1_a, siSUN1_b. siSUN1_c, and siSUN1_d. Bar, 10 µm. (b) Cells were transfected with indicated siRNA. SUN1 and β-tubulin protein expressions were analyzed. (c) Box-and-whiskers plots represent GM130 labeled Golgi complex area per cell. ** P < 0.01, compared with control cells. (d) Cells were transfected with siSUN1. After 24 h, cells were transfected with Myc-tagged mouse SUN1 and incubated for 24 h. The cells were then stained with anti-GM130 pAb and anti-Myc mAb. Bar, 10 µm. (e) HeLa cells were transfected with siSUN1 and stained with anti-GM130 mAb and anti-TGN46 pAb. Bar, 10 µm. (f) GFP-tsVSVG was transfected into control or SUN1-knockdown cells. After shifting to the permissive temperature, cells were incubated for 2.5 h. VSVG (green) and the Golgi complex (red) were detected using GFP and anti-GM130 mAb, respectively. (g) The ratio of VSVG intensity in the GM130 positive Golgi area to total VSVG in the cell is shown. The gray line with triangle and the black like with the circle represents control and SUN1-depleted cells, respectively.