Figure 2

SUN2 functions in dispersal of the Golgi complex. (a) Cells were transfected with a pool of siSUN2 or siNC and stained with anti-GM130 mAb (green) and anti-SUN2 pAb (red). Bar, 10 µm. Note that, siSUN2 contains four siRNAs, siSUN2_a, siSUN2_b. siSUN2_c, and siSUN2_d. (b) Cells were transfected with indicated siRNA. SUN2 and β-tubulin protein expressions were analyzed. (c) Box-and-whiskers plots represent GM130 labeled Golgi complex area in SUN2 depleted cells. (d) Cells were transfected with siSUN1 and siSUN2 or siNC. The cells were then stained with anti-SUN1 or anti-SUN2 pAb (red) and anti-GM130 mAb (green). Bar, 10 µm. (e) Box-and-whiskers plots represent GM130 labeled Golgi complex area in indicated cells. * P < 0.05, compared with siNC transfected cells. (f) Cells were transfected with siSUN1 and siSUN2. After 12 h, cells were transfected with Myc-tagged mouse SUN2 and incubated for 36 h. The cells were then stained with anti-Myc mAb and anti-GM130 pAb. Bar, 10 µm.