Figure 3

Nesprin-2 functions in dispersal of the Golgi complex but not in the steady state perinuclear Golgi structure. (a) Cells were transfected with siSUN1 and indicated siRNAs including siSYNE1, siSYNE2, or siSYNE3, which targets nesprin-1, nesprin-2, and nesprin-3, respectively. The cells were then stained with anti-GM130 mAb. Bar, 10 µm. (b, d) Box-and-whiskers plots represent GM130 labeled Golgi complex area. * P < 0.05. (c) Cells were transfected with indicated siRNA and stained with anti-GM130 mAb (green) and DAPI (red). (e) Cells were transfected with HA-tagged ΔN-SUN1 and stained with anti-HA mAb (red) and anti-Golgi pAb (green). (f) Box-and-whiskers plots represent GM130 labeled Golgi complex area in HA-tagged ΔN-SUN1 transfected or non-transfected cells.