Figure 5

SUN1 functionally associates with Golgi organization in vivo. (a) The cerebella from 30-day old male wild-type and Sun1-knockout mice were stained using anti-GM130 pAb and anti-calbindin (a Purkinje cell marker protein) mAb. Bar, 20 µm. The panels on the right are enlarged images of the indicated region in the adjacent panels on the left. (b) Golgi distribution was quantified using GM130 staining as the average percentage of total cellular fluorescence in each of the four quadrants (shown at left). The results were obtained from the quantification of 10 < Purkinje cells in each genotype. Data are presented as the mean ± sem, ** P < 0.01, compared with the same region in wild-type tissues.