Figure 3
UCP1 and UCP4 immunostaining in non-cortical regions of the harbor porpoise brain. In addition to examining the expression of UCP1 and UCP4 in the cerebral cortex of the brain of the harbor porpoise, we examined several other brain regions. In all regions we found neurons with distinct UCP1 immunoreactivity, with an intracellular staining pattern similar to that observed in the neurons of the cerebral cortex. The photomicrographs shown here depict UCP1 immunostaining in various non-cortical regions of the harbour porpoise brain, including the nucleus basalis, nucleus ellipticus, the substantia nigra (A9), and the nucleus subcoeruleus (A7d, its diffuse region). In addition, in all regions we found glial cells with distinct immunoreactivity to the UCP4 antibody. Interestingly, the density of glial cells immunopositive for UCP4 appears higher in the white matter than in the grey matter, reflecting the same proportional distribution of stained glia as when comparing the white and grey matter of the cerebral cortex. The photomicrographs shown here depict UCP4 immunostaining in various non-cortical regions of the harbour porpoise brain, including the striatum (P—putamen, ic—internal capsule), dorsal thalamus, ventral pons (VPO—ventral pontine nucleus, lfp—longitudinal fasciculus of pons) and the ventral medulla oblongata (io—portion of inferior olivary nuclear complex). Scale bar = 250 µm and applies to all.
