Figure 4
UCP4 Western blotting and immunostaining in cetartiodactyl cerebral cortex. While UCP4 was present in the cortical grey and white matter of all species, as evidenced in the Western blot at the top of the panel from the occipital cortex of all species studied, it was only found to be immunolocalized to glial cells in the cetaceans (See Figure S6 for full-length unedited Western immunoblots). Photomicrographs of Nissl-stained (purple colored images) and UCP4-immunostained (brown colored images) from cortical and subcortical white matter sections in a range of cetacean species. Note the presence of UCP4-immunoreactivity in approximately 30% of glial cells in the cerebral cortex and approximately 60% of glial cells in the white matter in all cetacean species (Table 1). The scale bar in the UCP4-immunostained section of Megaptera novaeangliae—white matter, equals 100 µm and applies to all photomicrographs. Pp—harbor porpoise, Phocoena phocoena; Ba—minke whale, Balaenoptera acutorostrata; Mn—humpback whale, Megaptera novaeangliae; Ct—blue wildebeest, Connochaetes taurinus; Ts—greater kudu, Tragelaphus strepsiceros; Dp—blesbok, Damaliscus pygargus; Ss—domestic pig, Sus scrofa; Cd—dromedary camel, Camelus dromedarius; Gm—sand gazelle, Gazella marica; Am—springbok, Antidorcas marsupialis; Sc—African buffalo, Syncerus caffer; Ha—river hippopotamus, Hippopotamus amphibius; Cn—Nubian ibex, Capra nubiana; Ta—nyala, Tragelaphus angasii; Rn—laboratory rat, Rattus norvegicus.
