Figure 2
U251 orthotopic implant produces contrast enhancing HGG xenograft intracranial tumor in mice. (A) Photograph of U251 implanted (arrow) in mouse brain before (Left) and after (Right) cranial window (dashed box) placement in surgical field (dotted lines) on POD0. A: anterior; P: posterior; L: left; R: right. (B) Maximum intensity projection (MIP) image (2 × 2 stitched tiles) across 0-300 µm below cranial window 30 s after intravenous injection of 3 kDa fluorescein-dextran on POD7. Yellow box: a single-tile MIP image at implant site. (C) T2-weighted (pre-contrast) and T1-weighted (pre- and post-contrast) MRI scans from a mouse with U251 glioma xenograft on POD15. Arrowhead: non-enhancing region within tumor. (D) MRI contrast of tumor against normal (Mean ± SEM) revealed significant statistical difference (*P < 0.001) between T1-weighted images with and without contrast injection. (E) Bioluminescence imaging (BLI) of glioma xenograft growth in mice over 30 days. (F) Tumor size measured by bioluminescence grew exponentially after a latent period. Dotted lines: standard error of the mean. Arrow: beginning of exponential growth.
