Figure 2 | Scientific Reports

Figure 2

From: Ablation of kynurenine 3-monooxygenase rescues plasma inflammatory cytokine levels in the R6/2 mouse model of Huntington’s disease

Figure 2

KMO protein levels are increased in R6/2 liver at 12 weeks of age and undetectable after Kmo ablation. (a) Two intercrosses were performed to generate the six desired genotypes: WT:KMO+/+ (WT), WT:KMO+/− (Kmo heterozygotes), WT:KMO−/− (Kmo homozygotes), R6/2:KMO+/+ (R6/2), R6/2:KMO+/− (R6/2 Kmo heterozygotes) and R6/2:KMO−/− (R6/2 Kmo homozygotes) for behavioural and molecular analyses. (b) Liver lysates from 12 week old mice for all six genotypes were immunoprobed for KMO by western blot with HSP90 as a loading control (n = 4/genotype) (c) Liver KMO levels were reduced in Kmo heterozygotes and ablated in Kmo knockout homozygotes in both R6/2 and WT mice. (d) Liver lysates from 12 week old WT and R6/2 mice were immunoprobed for KMO by western blot with HSP90 as a loading control (n = 8/genotype) (e) KMO levels were significantly elevated in R6/2 liver compared to WT littermates. Statistical analyses via one way ANOVA with Bonferroni post hoc correction or Student’s t-test; ***p ≤ 0.001. The test statistics, degrees of freedom and p values are provided in Supplementary Table S8 online. WT = wild type.

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