Figure 3

Transcript levels of KP pathway enzymes in response to the ablation of KMO in R6/2 and WT mice at 12 weeks of age. (a–c) Comparison of the levels of KP enzyme transcripts, as measured by qPCR, between WT and R6/2 mice heterozygous or homozygous knockout for Kmo at 12 weeks of age in liver, spleen, and peritoneal macrophages. (a) Liver Kmo transcript levels were reduced in WT and R6/2 mice heterozygous and homozygous for Kmo knockout. Liver transcript levels for Haao were restored in R6/2 mice heterozygous for Kmo. (b) Spleen Kmo transcript levels were reduced in both WT and R6/2 mice homozygous and heterozygous for Kmo knockout. Spleen transcript levels for Aadat were significantly increased in both WT and R6/2 mice homozygous for Kmo knockout. Spleen transcript levels for Haao were restored in R6/2 mice both homozygous and heterozygous for Kmo (c) Peritoneal macrophage Kmo transcript levels were reduced in WT and R6/2 mice heterozygous and homozygous for Kmo knockout. Kynu levels were restored in R6/2 mice homozygous for Kmo. Statistical analyses via one way ANOVA with Bonferroni post hoc correction; ***p ≤ 0.001, n = 4 per gender/genotype. The test statistic, degrees of freedom and p values for the ANOVA are provided in Supplementary Table S9 online. WT:KMO^+/+  = WT, WT:KMO^+/−  = Kmo heterozygotes, WT:KMO^−/−  = Kmo homozygotes, R6/2:KMO^+/+  = R6/2, R6/2:KMO^+/−  = R6/2 Kmo heterozygotes, R6/2:KMO^−/−  = R6/2 Kmo homozygotes, WT = wild type.