Figure 7
Kmo transcripts were visualised in Kupffer cells and eliminated in KMO knockout mice. (a) Kmo transcript levels in liver were visualised using RNAScope and localised to macrophagic Kupffer cells by co-immunohistochemistry with the F4/80 antibody. The absence of a Kmo signal in the Kmo knockout sections demonstrated that the Kmo probe was specific (3rd and 6th rows). Activation of Kupffer macrophages was more pronounced in liver from R6/2 mice at 12 weeks of age than their WT littermates. Ablation of KMO decreased the F4/80 signal in R6/2 mice to WT levels. The panels on the right are zoomed images of the area on interest in the merged panels. (b) Fluorescence intensity for F4/80 signal (arbitrary units) were analysed using image J, based on 30 images per animal. The test statistic, degrees of freedom and p values for the ANOVA are provided in Supplementary Table S13 online. ***p < 0.001, n = 3 per genotype. WT:KMO+/+ = WT, WT:KMO+/− = Kmo heterozygotes, WT:KMO−/− = Kmo homozygotes, R6/2:KMO+/+ = R6/2, R6/2:KMO+/− = R6/2 Kmo heterozygotes, R6/2:KMO−/− = R6/2 Kmo homozygotes, WT = wild type.
