Table 1 Comparison of effects of the two methods for sampling microbes on the surface of tobaccos.

From: The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos

Samples

Sequence characterized amplified region

Test result

Test conclusion

Surface 1 of fresh tobacco leaves

S5-16SV4

Unqualified

Unsuitable

Surface 2 of fresh tobacco leaves

S5-16SV4

Qualified

Suitable

Surface 1 of tobacco leaves in the later yellowing period

S5-16SV4

Unqualified

Unsuitable

Surface 2 of tobacco leaves in the later yellowing period

S5-16SV4

Qualified

Suitable

  1. 1 and 2 represent the first and second methods for sampling microbes on the surface of tobaccos, respectively.