Figure 1
From: Cannabidiol induces autophagy via ERK1/2 activation in neural cells
CBD modulates cell death in a concentration and time-dependent manner. SH-SY5Y cells were treated with different CBD concentrations and assessed using the Propidium Iodide cell cycle assay. (A) Representative histogram of the sub-G0/G1 population (an indicative of cell death) of untreated cells (CTR), cells treated with 1, 2, 5, 10, 25, 50 or 100 µM of CBD and 1 µM of staurosporine (STS) for 24 and 48 h. (B) The percentage of SH-SY5Y cells in the sub-G0/G1 phase of the cell cycle after 24 h of treatment with the different CBD concentrations. The results showed that 50 and 100 µM of CBD increased the percentage of cells in the sub-G0/G1 phase. (C) The percentage of SH-SY5Y cells in the sub-G0/G1 phase of the cell cycle after 48 h of treatment with the different CBD concentrations. The results showed that 25, 50 and 100 µM of CBD increased the percentage of cells in the sub-G0/G1 phase. Data are expressed as the mean ± S.E.M. Two-way ANOVA (F8,88 = 22.764) followed by Dunnett’s post-hoc test; ***p < 0.001 relative to the CTR group.
