Figure 1
Single isotherms may lack range and resolution to discriminate self-association from nonideality. (A) z-average diffusion coefficients of the most strongly self-associating molecule (mAb C) measured by DLS (circles), with best-fit linear regression in blue, resulting in kD,op = −40.1 mL/g. Shown in magenta is the best-fit self-association model when jointly fitting the Mw isotherm from SLS (inset); this results in a best-fit kD of − 6.6 mL/g. (B) z-average diffusion coefficients from DLS for mAb B that does not exhibit self-association (circles), with linear regression (blue line) jointly modeling the DLS data and the Mw isotherm from SLS (inset) as a single nonideal species with kD,op = −4.1 mL/g. Diffusion coefficients are reported in units of Ficks, with 1 F = 10−11 m2/s.
