Figure 2
Isotherms sampled at experimental concentrations will not represent limiting slopes. Shown are isotherms of weight-average sedimentation coefficient of a protein in monomer–dimer self-association equilibrium with different equilibrium constants (color code in legend) with nonideality as predicted by the modified Richardson–Zaki expression by Fiore et al.20 assuming moderate shape asymmetry (solid lines). Circles represent potential measurements at concentrations spaced across experimentally accessible range. Slopes in the limit of low concentrations are depicted as dashed lines.
